Hershey and Chase’s Experiment

Hershey and Chase’s Experiment
Introduction
1n 1952, Hershey and chase performed experiment to proof that DNA is a hereditary material.
Experiment at Material
Hershey and chase labeled protein coat and DNA of Bacteriophage separately. Protein coat was labeled with radioactive sulphur and DNA with radioactive phosphorus. These two viruses used to attack bacterial cells.
Step of Experiment
1. Hershey and chase observed that if cultures of bacteriophage are labeled with radioactive phosphorus or with sulphur.
2. Bacteriophage is ruptured, the DNA is released and treated with deoxyribsonucleus, the DNA breaks up into fragments in the solution.
3. The empty protein coats of the ruptured membrane appear as coats all the phosphorus and sulphur were made to inject bacteria and multiply by the help of special technique, all the sulphur labeled protein were removed.
4. The new phage formed contained only phosphorus indicating the presence of DNA molecule.
Conclusion
The conclusion appears similar to the transforming principle in bacteria, showing that DNA is the genetic material in phage, transmitted from one generation to the next.